PaxView HPV20 GENOTYPING MPCR-ULFA KIT
PaxView® HPV 20 Genotyping MPCR-ULFA Kit is an in vitro diagnostic kit designed for the detection and genotyping of HPV in cervical swab specimens. The kit utilizes multiplex PCR and DNA hybridization to detect and identify 20 different HPV genotypes (6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 70, 73).
[Principles of the Procedure]
PaxView® HPV 20 Genotyping MPCR-ULFA Kit uses MPCR-ULFA (Multiplex PCR - Universal Lateral Flow Assay) technology for the qualitative detection and identification of HPV genotypes.
The kit is comprises of MPCR kit for the multiplex PCR amplification of HPV DNA and ULFA kit for the detection of amplified PCR products.
MPCR kit includes PCR premix and primer mix for specific amplification of 20 HPV genotypes. Forward primers for the amplification include the nucleotide sequence complementary to the universal DNA probes immobilized on the nitrocellulose membrane of the cartridge. Reverse primers are biotinylated for the binding to streptavidin-conjugated gold nanoparticles for visualization. The PCR products move on the membrane along with running buffer and hybridize to the DNA probe on the membrane. When amplifying the DNA hybridizes to the probe, the red line will appears on the test line if HPV is present. If not, only Control lines appears.